A trove of information exists about Abraham Lincoln's funeral, which drew millions of mourners during a two-week railway procession across the Northern states.

But until now, the precise color of the president's railcar had been lost to history.

With the 2015 sesquicentennial of Lincoln's death approaching, interest in it is rising, and with new tools, researchers at the University of Arizona have turned their attention to one of the last remaining mysteries about what was "perhaps the largest traditional funeral in American history," says Wayne Wesolowski.

Wesolowski, a chemist and model train maker, was director of the Lincoln Train Project at Benedictine University near Chicago for 10 years. In 1995, he completed a years-long project of building a scale model of Lincoln's car, the locomotive and hearse and horses, all together measuring nearly 15 feet in length.

After 30 years as a chemistry professor at Benedictine, Wesolowski retired to Tucson, and continues to teach as a chemistry lecturer at the UA.

A Chicago group known as the Lincoln Funeral Car Project approached Wesolowski to consult on their efforts to build a full-size version of Lincoln's funeral car, intending to trace as closely as possible the funeral route for the 150th anniversary. An obvious question: what color to paint the new replica?

However, no color photographs, no color lithographs and no contemporary color paintings exist of Lincoln's private car, named "The United States." Newspaper accounts from the time describe the color as both "rich chocolate brown" and "claret red." But "chocolate" in 1865 was strictly a drink, very different from the milk chocolate we know today, so the two descriptions are compatible.

The car burned in a fire in 1911, having been sold at auction to Union Pacific after the funeral and passing through several private hands afterward. Just one artifact of exterior wood survived, and after years of searching, Wesolowski acquired a pencil sized piece of trim.

Using three separate labs at the UA – inchemistry/biochemistry (Brook Beam, Keck Imaging Center), art (Karen Zimmermann, Jack Sinclair Letterpress Studio) and the Arizona State Museum – Wesolowski set about investigating for the true color.

And with the help of Nancy Odegaard, conservator and head of the preservation division, comparing layers of microscopic paint chips from the original car to national color standards, Wesolowski at last found the true original color, which he describes as a dark maroon, darker, but not too far off of what he'd painted his model.

The effort at historical exactness reflects on how deeply the country mourned Lincoln's death. In early 1865, the United States Military Railroad delivered Lincoln a private railroad car for presidential use. But Lincoln never used the car alive. His presidential funeral procession left Washington on April 21, 1865, closely retracing the route Lincoln traveled as president-elect in 1861, bypassing cities with a large number of Southern sympathizers.

"It was a procession of mourning and without TV or radio, the only way to participate was to leave the farm, close the store and come trackside," Wesolowski says. "Just being there was so important. It was a colossal event."

Millions of Americans – an estimated one-third of the Northern population – came in person to see the funeral. In New York and Chicago, the crowds topped a half-million. In the countryside, people lined the tracks just to glimpse the train as it passed, similar to the Robert Kennedy funeral train.

"It was a political event. It was a social event. It was a catharsis. The man who said in victory, 'Malice toward none,' was dead," Wesolowski says. "There is now a chance to re-create a little of that history."

The greatest battle in Earth's history has been going on for hundreds of millions of years, isn't over yet, and until now no one knew it existed, scientists reported today in the journal Nature.

In one corner is the Earth's most abundant organism: SAR11, an ocean-living bacterium that survives where most other cells would die and plays a major role in the planet's carbon cycle. It had been theorized that SAR11 was so small and widespread that it must be invulnerable to attack.

In the other corner, and so strange looking that scientists previously didn’t even recognize what they were, are "Pelagiphages," viruses now known to infect SAR11 and routinely kill millions of these cells every second. And how this fight turns out is of more than casual interest, because SAR11 has a huge effect on the amount of carbon dioxide that enters the atmosphere, and the overall biology of the oceans.

"There's a war going on in our oceans, a huge war, and we never even saw it," said Stephen Giovannoni, a professor of microbiology at Oregon State University. "This is an important piece of the puzzle in how carbon is stored or released in the sea."

The paper in Nature describes four previously unknown viruses that infect SAR11. To prove the viruses were as abundant as their hosts, OSU teamed up with researchers at the University of Arizona’s Tucson Marine Phage Research Lab, led by Matthew Sullivan, who had developed accurate methods for measuring viral diversity in nature.

The analysis shows that the new viruses – like their hosts – are the most abundant on record. Sullivan is an assistant professor in the UA's department of ecology and evolutionary biology with a joint appointment in the department of molecular and cellular biology.

"The methods and datasets developed by Matt's lab at the University of Arizona will make it possible for a generation of marine microbiologists to more accurately determine viral distributions in nature," Giovannoni said. "It's a major step forward for the field."

Giovannoni's group discovered the Pelagiphage viral families by using "old-fashioned" research methods, growing the cells and viruses in a laboratory, instead of the tools of modern genomics, and found the new type of virus.

"Because they are so new, these viruses were virtually unrecognizable to us based on their DNA," Giovannoni said.

"The viruses themselves, of course, appear to be just as abundant as SAR11," he added. "Our colleagues at the UA demonstrated this with new technologies they developed for measuring viral diversity."

Sullivan explained the method for discovering viruses in the oceans based on their genomes his group developed over four years is at least 1,000 times more accurate than previous methods.

Much of the analyses and datasets enabling the discovery of the viruses were generated by Bonnie Hurwitz, a former graduate student of Sullivan’s who is now program director of health informatics at the Arizona Health Sciences Center, with help from research scientist Bonnie Poulos and other members of the Tucson Marine Phage Laboratory.

Their work, soon to be published in the open-access journal PLoS One, resulted in the Pacific Ocean Virus dataset. This dataset, Sullivan explained, is the viral equivalent of the Global Ocean Sampling Expedition by former human genome researcher J. Craig Venter, who sailed across the world's oceans sampling, sequencing and analyzing the DNA of the microorganisms living in these waters.

The new findings on SAR11 disprove the theory that the bacteria are immune to viral predation, Giovannoni and his co-authors said.

"In general, every living cell is vulnerable to viral infection," said Giovannoni, who first discovered SAR11 in 1990. "What has been so puzzling about SAR11 was its sheer abundance, there was simply so much of it that some scientists believed it must not get attacked by viruses."

What the new research shows, Giovannoni said, is that SAR11 is competitive, good at scavenging organic carbon, and effective at changing quickly to avoid infection. Because of this, it thrives and persists in abundance even though it's constantly being killed by the new viruses that have been discovered.

SAR11 has several unique characteristics, including the smallest known genetic structure of any independent cell. Through sheer numbers, this microbe has a huge role in consuming organic carbon, which it uses to generate energy while producing carbon dioxide and water in the process. SAR11 recycles organic matter, providing the nutrients needed by algae to produce about half of the oxygen that enters Earth's atmosphere every day.

This carbon cycle ultimately affects all plant and animal life on Earth.

"Because of their huge numbers, these cells are an important part of models that aim to understand and predict long-term patterns of carbon sequestration in the oceans," Giovannoni said.

"Microbes fix half of the oxygen in the air we breathe and drive every biogeochemical cycle that fuels Earth," Sullivan added. "Most of this happens in the oceans, and it turns out the most abundant microbes on the planet are the SAR11 bacteria."

Other contributors to this research included scientists at the University of California, San Diego’s National Center for Microscopy and Imaging Research and the Monterey Bay Aquarium Research Institute, which provided opportunity to sample viruses from nature.

Funding for the methods development and genomic sequencing was provided by the Gordon and Betty Moore Foundation Marine Microbiology Initiative and the Department of Energy Joint Genome Institute.